Composite

Part:BBa_K1811777

Designed by: Samuel Keating   Group: iGEM15_UMBC-Maryland   (2015-07-29)

CUP1 copper resistance gene with RBS and promoter

Metallothionein is a copper binding protein native to S. cerevisiae that gives cells resistance to copper toxicity by binding 11 copper atoms. It prevents copper from reacting to form toxic molecules when bound and detoxifies free radicals. The part contains the metallothionein gene CUP1 (BBa_M45090), a strong constituitive promoter (BBa_J23100), and an RBS (BBa_B0034). The CUP1 gene was previously submitted by the 2014 Utah State team, but this part was created from a single G Block assembled into the plasmid pSB1C3.

800px-UMBCRet1OD.jpg
UMBCRet1Cu.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None